Antigenic maps including laboratory-made mutants with substitutions at positions 417, 452, 484, and 501. The same antigenic map as shown in Fig. 2 but additionally showing laboratory-made mutants. A) Variants with substitutions N501Y, E484K, E484Q, and L452R+E484K in the background of D614G; D614G+L452R is not shown since it was titrated against only D614G sera, so its position cannot be determined. B) Variants with the T/N417K substitution in the background of P.1 and B.1.351 respectively, and K417N in the background of B.1.429 and B.1.617.1. C) BA.1 with the substitution A484K. The map in panel C is in 3D to highlight the antigenic differences between BA.1, BA.1+A484K, and BA.4/BA.5. The 2D version of panel C is shown in fig. S27. Lines link the antigenic position of the root virus to that of the laboratory-generated variant.